Targeted Single Cell BCR and TCR Sequencing for Paired Chain Profiling

 

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Explore Single-Cell V(D)J Sequencing for Paired TCR/BCR Chain Insights

When RNA is extracted for bulk repertoire sequencing, information related to the cognate pairing of BCR and TCR chains is lost. iRepertoire's iPair is the first consumer-based single-cell immune sequencing service specifically designed for capturing physically paired variable regions in T cells and B cells.

Our customizable service can also provide phenotypic analysis coupled with the single cell VDJ data, providing specific and detailed immune insight of your samples.

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Choose iRepertoire’s iPair powerful and flexible technology for your next experiment

  • Cost-effective Single cell VDJ sequencing
  • Fully customizable service
  • Scientific support from expert immunologists
  • Detailed BCR and TCR sequencing analysis
  • Includes basic bioinformatic analysis with a user-friendly GUI
  • Compatible with human or mouse samples
  • Track VDJ recombination in B or T cells
  • Full CDR3 analysis
  • Alpha/beta TCR pairing
  • Gamma/delta TCR pairing
  • IgH kappa/lambda pairing
  • Option to add phenotyping with Immunosight
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Supportive software makes analysis simpler. Find the answers in your sequencing data with the iPair Analyzer

  • Complementary data analysis
  • Well-by-well GUI lets you filter by read depth, unique CDR3s, chain usage, or V/D/J usage
  • Parse out significant data points to streamline downstream analysis
  • Generate heatmaps and T-SNE plots
  • Easily compare single-chain comparison analysis to bulk sequencing data
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Let us help plan your project. Get a free consultation with one of our experts.


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CASE STUDY

Applications for single cell immune sequencing

  • Reverse engineering
  • Full BCR/TCR identity and chain usage
  • CAR-T development
  • Antibody identification
  • Lymphocyte tracking
  • Analyze rare cell populations or cell line sequencing