Capture paired BCR/TCR chains with single cell immune sequencing

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Supportive software makes analysis simpler. Find the answers in your sequencing data with the iPair AnalyzerTM

  • Complimentary data analysis
  • Well-by-well GUI lets you filter by read depth, unique CDR3s, chain usage, or V/D/J usage
  • Parse out significant data points to streamline downstream analysis
  • Generate heatmaps and T-SNE plots
  • Easily compare single chain comparison analysis to bulk sequencing data
B Cell Learning Center

Let us help plan your project. Get a free consultation with one of our experts.

When RNA is extracted for bulk repertoire sequencing, information related to the cognate pairing of BCR and TCR chains is lost. iRepertoire’s technology, iPair, is the first consumer-based single cell immune sequencing service specifically designed for capturing physically paired variable regions in T cells and B cells.

Our customizable service can also provide phenotypic analysis coupled with the single cell VDJ data, providing specific and detailed immune insight of your samples.

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Choose iRepertoire’s iPair powerful and flexible technology for your next experiment

  • Extremely cost-effective
  • Fully customizable service
  • Scientific support from expert immunologists
  • Multiple applications to fit your project needs
  • Includes basic bioinformatic analysis with user-friendly GUI
  • Compatible with human or mouse samples
  • Track VDJ recombination in B or T cells
  • Full CDR3 analysis
  • Alpha/beta TCR pairing
  • Gamma/delta TCR pairing
  • IgH kappa/lambda pairing
  • Option to add phenotyping with iPair+

CASE STUDY

Applications for single cell immune sequencing

  • Reverse engineering
  • Full BCR/TCR identity and chain usage
  • CAR-T development
  • Antibody identification
  • Lymphocyte tracking
  • Analyze rare cell populations or cell line sequencing